4) OBL – Publications

Orthopaedic Biotechnology Lab

Director: Dr. Laura De Girolamo

Our Publications

Topic 1: Tissue Specific Progenitors and MSCs



Perteghella S, Martella E, de Girolamo L, Perucca Orfei C, Pierini M, Fumagalli V, Pintacuda DV,

Chlapanidas T, Viganò M, Faragò S, Torre ML, Lucarelli E. Fabrication of Innovative Silk/Alginate

Microcarriers for Mesenchymal Stem Cell Delivery and Tissue Regeneration. Int J Mol Sci. 2017 Aug




Viganò M, Perucca Orfei C, Colombini A, Stanco D, Randelli P, Sansone V, de Girolamo L.

Different culture conditions affect the growth of human tendon stem/progenitor cells (TSPCs) within

a mixed tendon cells (TCs) population. J Exp Orthop. 2017 Dec;4(1):8.



Petrangeli E, Coroniti G, Brini AT, de Girolamo L, Stanco D, Niada S, Silecchia G, Morgante E,

Lubrano C, Russo MA, Salvatori L. Hypoxia promotes the inflammatory response and stemness

features in visceral fat stem cells from obese subjects. J Cell Physiol. 2016;231(3):668-7.



de Girolamo L, Niada S, Arrigoni E, Di Giancamillo A, Domeneghini C, Dadsetan M, Yaszemski

MJ, Gastaldi D, Vena P, Taffetani M, Zerbi A, Sansone V, Peretti GM, Brini AT. Repair of

osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived

mesenchymal stem cells. Regen Med. 2015;10(2):135-51.



Stanco D, Viganò M, Perucca Orfei C, Di Giancamillo A, Peretti GM, Lanfranchi L, de Girolamo

  1. Multidifferentiation potential of human mesenchymal stem cells from adipose tissue and

hamstring tendons for musculoskeletal cell-based therapy. Regen Med. 2015;10(6):729-43.



Stanco D, Viganò M, Orfei CP, DI Giancamillo A, Thiebat G, Peretti G, de Girolamo L. In vitro

characterization of stem/progenitor cells from semitendinosus and gracilis tendons as a possible new

tool for cell-based therapy for tendon disorders. Joints. 2015;2(4):159-68.



Torre ML, Lucarelli E, Guidi S, Ferrari M, Alessandri G, de Girolamo L, Pessina A, Ferrero I. Exvivo

expanded mesenchymal stromal cell minimal quality requirements for clinical application. Stem

Cells Dev. 2015;24(6):677-85.



De Luca P, Arrigoni C, Gilardi M, Previdi S, Broggini M, Moretti M. Direct but not indirect co-culture with osteogenically differentiated human bone marrow stromal cells increases RANKL/OPG ratio in human breast cancer cells generating bone metastases. Molecular Cancer. 2014; 13(1): 1-6.



Marmotti A, de Girolamo L, Bonasia DE, Bruzzone M, Mattia S, Rossi R, Montaruli A, Dettoni F,

Castoldi F, Peretti G. Bone marrow derived stem cells in joint and bone diseases: a concise review. Int

Orthop. 2014;38(9):1787-801.



Lopa S, Colombini A, Stanco D, de Girolamo L, Sansone V, Moretti M. Donor-matched

mesenchymal stem cells from knee infrapatellar and subcutaneous adipose tissue of osteoarthritic

donors display differential chondrogenic and osteogenic commitment. Eur Cell Mater. 2014; 27:298-




Lopa S, Colombini A, Sansone V, Preis FW, Moretti M. Influence on chondrogenesis of human osteoarthritic chondrocytes in co-culture with donor-matched mesenchymal stem cells from infrapatellar fat pad and subcutaneous adipose tissue. Int J Immunopathol Pharmacol. 2013;26(1 Suppl):23-31.



Lopa S, Mercuri D, Colombini A, De Conti G, Segatti F, Zagra L, Moretti M. Orthopedic bioactive implants: Hydrogel enrichment of macroporous titanium for the delivery of mesenchymal stem cells and strontium. J Biomed Mater Res A. 2013;101(12):3396-403.



Arrigoni E, de Girolamo L, Di Giancamillo A, Stanco D, Dellavia C, Carnelli D, Campagnol M,

Domeneghini C, Brini AT. Adipose-derived stem cells and rabbit bone regeneration:

histomorphometric, immunohistochemical and mechanical characterization. J Orthop Sci.




de Girolamo L, Stanco D, Salvatori L, Coroniti G, Arrigoni E, Silecchia G, Russo MA, Niada S,

Petrangeli E, Brini AT. Stemness and osteogenic and adipogenic potential are differently impaired in

subcutaneous and visceral adipose derived stem cells (ASCs) isolated from obese donors. Int J

Immunopathol Pharmacol. 2013;26(1 Suppl):11-21.



Arrigoni E, Niada S, Ferreira LM, de Girolamo L, Brini AT. Two bone substitutes analyzed in

vitro by porcine and human adipose-derived stromal cells. Int J Immunopathol Pharmacol. 2013;26(1




Sacerdote P, Niada S, Franchi S, Arrigoni E, Rossi A, Yenagi V, de Girolamo L, Panerai AE, Brini

  1. Systemic administration of human adipose-derived stem cells reverts nociceptive hypersensitivity

in an experimental model of neuropathy. Stem Cells Dev. 2013;22(8):1252-63.



Lopa S, de Girolamo L, Arrigoni E, Stanco D, Rimondini L, Baruffaldi Preis FW, Lanfranchi L,

Ghigo M, Chiesa R, Brini AT. Enhanced biological performance of human adipose-derived stem cells

cultured on titanium-based biomaterials and silicon carbide sheets for orthopaedic applications. J

Biol Regul Homeost Agents. 2011;25(2 Suppl):S35-42.



Conforti E, Arrigoni E, Piccoli M, Lopa S, de Girolamo L, Ibatici A, Di Matteo A, Tettamanti G,

Brini AT, Anastasia L. Reversine increases multipotent human mesenchymal cells differentiation

potential. J Biol Regul Homeost Agents 2011;25(2 Suppl):S25-33.



de Girolamo L, Arrigoni E, Stanco D, Lopa S, Addis A, Borgonovo S, Dellavia C, Domeneghini C,

Brini AT. Role of autologous rabbit adipose-derived stem cells in the early phases of the repairing

process of critical bone defects. J Orthop Research 2011;29:100-8.



Lopa S, Colombini A, de Girolamo L, Sansone V, Moretti M. New strategies in cartilage tissue

engineering for osteoarthritic patients: infrapatellar fat pad as an alternative source of progenitor

cells. Journal of Biomaterial and Tissue Engineering 2011;1:1-9.



Quirici N, Scavullo C*, de Girolamo L*, Lopa S, Arrigoni E, Lambertenghi Deliliers G, Brini AT.

Anti-L-NGFR and -CD34 Monoclonal Antibodies identify multipotent mesenchymal stem cells in

human adipose tissue. Stem Cells Dev 2010;19(6):915-25. (*equal contribution to the work).



Arrigoni E, Lopa S, de Girolamo L, Stanco D, Brini AT. Isolation, characterization and osteogenic

differentiation of adipose-derived stem cells: from small to large animal models. Cell Tissue Res 2009;




de Girolamo L, Lopa S, Arrigoni E, Sartori MF, Baruffaldi Preis FW, Brini AT. hASCs (human

Adipose-derived Stem Cells) isolated from young and elderly women: study on their differentiation

potential and scaffold interaction during osteogenic differentiation. Cytotherapy 2009;11(6):793-803.



de Girolamo L, Sartori MF, Arrigoni E, Rimondini L, Albisetti W, Weinstein RL, Brini AT. Human

adipose-derived stem cells as future tools in tissue regeneration: osteogenic differentiation and cellscaffold

interaction. Int J Artif Organs 2008 ;31(6):467-79.



de Girolamo L, Sartori MF, Albisetti W, Brini AT. Osteogenic differentiation of human adiposederived

stem cells: comparison of two different inductive media. J Tissue Eng Regen Med 2007;1:154-



Topic 2: MSCs derived products: Microvescicles and Secretome



de Girolamo L, Lucarelli E, Alessandri G, Avanzini MA, Bernardo ME, Biagi E, Brini AT, D’Amico

G, Fagioli F, Ferrero I, Locatelli F, Maccario R, Marazzi M, Parolini O, Pessina A, Torre ML, Italian

Mesenchymal Stem Cell Group. Mesenchymal stem/stromal cells: a new ”cells as drugs” paradigm.

Efficacy and critical aspects in cell therapy. Curr Pharm Des. 2013;19(13):2459-73.


Topic 3: Biophysical Stimulations



Viganò M, Sansone V, d’Agostino MC, Romeo P, Perucca Orfei C, de Girolamo L. Mesenchymal

stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal

disorders. J Orthop Surg Res. 2016 Dec 16;11(1):163.



Randelli P, Menon A, Ragone V, Creo P, Montrasio UA, Perucca Orfei C, Banfi G, Cabitza P, Tettamanti G, Anastasia L. Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study. BMC Complement Altern Med. 2016 Aug 18;16:293.



de Girolamo L, Viganò M, Galliera E, Stanco D, Setti S, Marazzi MG, Thiebat G, Corsi Romanelli

MM, Sansone V. In vitro functional response of human tendon cells to different dosages of low frequency

pulsed electromagnetic field. Knee Surg Sports Traumatol Arthrosc. 2014 Jun 23(11):3443-53.



Catalano MG, Marano F, Rinella L, de Girolamo L, Bosco O, Fortunati N, Berta L, Frairia R.

Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of

adipose-derived stem cells into osteoblast-like cells. J Tissue Eng Regen Med. 2014 Jun 11(2):390-399.



Adravanti P, Nicoletti S, Setti S, Ampollini A, de Girolamo L. Effect of pulsed electromagnetic field

therapy in patients undergoing total knee arthroplasty: a randomised controlled trial. Int Orthop.




de Girolamo L, Stanco D, Galliera E, Viganò M, Lovati AB, Marazzi MG, Romeo P, Sansone V.

Soft-Focused Extracorporeal Shock Waves Increase the Expression of Tendon-Specific Markers and

the Release of Anti-inflammatory Cytokines in an Adherent Culture Model of Primary Human Tendon

Cells. Ultrasound Med Biol. 2014 Jun;40(6):1204-15.



de Girolamo L, Stanco D, Galliera E, Viganò M, Colombini A, Setti S, Vianello E, Corsi Romanelli

MM, Sansone V. Low frequency pulsed electromagnetic field affects proliferation, tissue-specific gene

expression, and cytokines release of human tendon cells. Cell Biochem Biophys. 2013;66(3):697-708.



Sansone V, D’ Agostino MC, Bonora C, Sizzano F, de Girolamo L, Romeo P. Early angiogenic

response to shock waves in a three-dimensional model of human microvascular endothelial cell

culture (HMEC-1). J Biol Regul Homeost Agents. 2012;26(1):29-37.


Topic 4: Biochemical and Genetic markers



Cauci S, Viganò M, de Girolamo L, De Luca P, Perucca Orfei C, Banfi G, Lombardi G, Brayda-

Bruno M, Colombini A. High Levels of Circulating Type II Collagen Degradation Marker (CTx-II) Are

Associated with Specific VDR Polymorphisms in Patients with Adult Vertebral Osteochondrosis. Int J

Mol Sci. 2017 Sep 29;18(10).



Brayda-Bruno M, Viganò M, Cauci S, Vitale JA, de Girolamo L, De Luca P, Lombardi G, Banfi G,

Colombini A. Plasma vitamin D and osteo-cartilaginous markers in Italian males affected by

intervertebral disc degeneration: Focus on seasonal and pathological trend of type II collagen

degradation. Clin Chim Acta. 2017 May 22;471:87-93.



Colombini A, Brayda-Bruno M, Lombardi G, Croiset SJ, Ceriani C, Buligan C, Barbina M, Banfi G, Cauci S. BsmI, ApaI and TaqI Polymorphisms in the Vitamin D Receptor Gene (VDR) and Association with Lumbar Spine Pathologies: An Italian Case-Control Study.PLoS One. 2016;11(5):e0155004.



Sansoni V, Perego S, Colombini A, Banfi G, Brayda-Bruno M, Lombardi G. Interplay between low plasma RANKL and VDR-FokI polymorphism in lumbar disc herniation independently from age,body mass, and environmental factors: a case-control study in the Italian population.Eur Spine J. 2015;25(1):192-9.



Colombini A, Brayda-Bruno M, Ferino L, Lombardi G, Maione V, Banfi G, Cauci S. Gender differences for FokI VDR polymorphism and conventional non-genetic risk factors in association with lumbar spine pathologies in an Italian case-control study.Int J Mol Sci. 2015;16(2):3722-39.



Colombini A, Brayda-Bruno M, Lombardi G, Croiset SJ, Vrech V, Maione V, Banfi G, Cauci S. FokI Polymorphism in the vitamin D receptor gene (VDR) and its association with lumbar spine pathologies in the italian population: a case-control study. PLoS One. 2014;9(5):e97027.



Colombini A, Cauci S, Lombardi G, Lanteri P, Croiset S, Brayda-Bruno M, Banfi G. Relationship between vitamin D receptor gene (VDR) polymorphisms, vitamin D status, osteoarthritis and intervertebral disc degeneration. J Steroid Biochem Mol Biol. 2013;138C:24-40.



Colombini A, Lanteri P, Lombardi G, Grasso D, Recordati C, Lovi A, Banfi G, Bassani R, Brayda-Bruno M. Metabolic effects of vitamin D active metabolites in monolayer and micromass cultures of nucleus pulposus andannulus fibrosus cells isolated from human intervertebral disc. Int J Biochem Cell Biol. 2012;44(6):1019-30.



Colombini A, Lombardi G, Corsi MM, Banfi G. Pathophysiology of the human intervertebral disc. Int J Biochem Cell Biol. 2008; 40(5):837-42.


Topic 5: Testing of biological products for regenerative medicine



Albano D, Messina C, Usuelli FG, de Girolamo L, Grassi M, Maccario C, Bignotti B, Tagliafico A,

Sconfienza LM. Magnetic resonance and ultrasound in achilles tendinopathy: Predictive role and

response assessment to platelet-rich plasma and adipose-derived stromal vascular fraction injection.

Eur J Radiol. 2017 Oct;95:130-135.



Usuelli FG, Grassi M, Maccario C, Vigano’ M, Lanfranchi L, Alfieri Montrasio U, de Girolamo L.

Intratendinous adipose-derived stromal vascular fraction (SVF) injection provides a safe, efficacious

treatment for Achilles tendinopathy: results of a randomized controlled clinical trial at a 6-month

follow-up. Knee Surg Sports Traumatol Arthrosc. 2017 Mar 1 doi: 10.1007/s00167-017-4479-9. [Epub ahead of print]



Usuelli FG, Grassi M, Manzi L, Guarrella V, Boga M, de Girolamo L. Treatment of osteochondral

lesions of the talus with autologous collagen-induced chondrogenesis: clinical and magnetic

resonance evaluation at one-year follow-up. Joints. 2016 Aug 18;4(2):80-6.



de Girolamo L, Kon E, Filardo G, Marmotti AG, Soler F, Peretti GM, Vannini F, Madry H,

Chubinskaya S Regenerative approaches for the treatment of early OA. Knee Surg Sports Traumatol

Arthrosc. 2016 Jun;24(6):1826-35.



Randelli P, Menon A, Ragone V, Creo P, Bergante S, Randelli F, de Girolamo L, Alfieri Montrasio

U, Banfi G, Cabitza P, Tettamanti G, Anastasia L. Lipogems product treatment increases the

proliferation rate of human tendon stem cells without affecting their stemness and differentiation

capability. Stem Cell Intern, 2016:4373410.



Colombini A, Lopa S, Ceriani C, Lovati AB, Croiset SJ, Di Giancamillo A, Lombardi G, Banfi G, Moretti M. In Vitro Characterization and In Vivo Behavior of Human Nucleus Pulposus and Annulus Fibrosus Cells in Clinical-Grade Fibrin and Collagen-Enriched Fibrin Gels.Tissue Eng Part A. 2015;21(3-4):793-802.



Colombini A, Ceriani C, Banfi G, Brayda-Bruno M, Moretti M. Fibrin in Intervertebral Disc Tissue Engineering. Tissue Eng Part B Rev. 2014;20(6):713-21.



Volpi P, Bait C, Quaglia A, Redaelli A, Prospero E, Cervellin M, Stanco D, de Girolamo L.

Autologous collagen-induced chondrogenesis technique (ACIC) for the treatment of chondral lesions

of the talus. Knee Surg Sports Traumatol Arthrosc. 2014;22(6):1320-6.



Gille J, Behrens P, Volpi P, de Girolamo L, Reiss E, Zoch W, Anders S. Outcome of Autologous

Matrix Induced Chondrogenesis (AMIC) in cartilage knee surgery: data of the AMIC Registry. Arch

Orthop Trauma Surg. 2013;133(1):87-93.



de Girolamo L, Quaglia A, Bait C, Cervellin M, Prospero E, Volpi P. Modified autologous matrixinduced

chondrogenesis (AMIC) for the treatment of a large osteochondral defect in a varus knee: a

case report. Knee Surg Sports Traumatol Arthrosc. 2012;20(11):2287-90.



Dolegowska B, Lubkowska A, de Girolamo L. Platelet lipodimic. Journal of Biological Regulators

& Homeostatic Agents. 2012;26(2S):23-34.



Stanco D, Viganò M, Croiset SJ, de Girolamo L. Application and limits of platelet-rich plasma in

sports related injuries. Journal of Biological Regulators & Homeostatic Agents. 2012;26(2S):53-62.



Cervellin M, de Girolamo L, Bait C, Denti M, Volpi P. Autologous platelet-rich plasma gel to

reduce donor site morbidity after patellar tendon graft harvesting for anterior cruciate ligament

reconstruction: a randomized, controlled clinical study. Knee Surg Sports Traumatol Arthrosc,




de Girolamo L, Bertolini G, Cervellin M, Sozzi G, Volpi P. Treatment of chondral defects of the

knee with one step matrix-assisted technique enhanced by autologous concentrated bone marrow: In

vitro characterisation of mesenchymal stem cells from iliac crest and subchondral bone. Injury